Proxcys B.V. is the world's primary producer of high performance low pressure cGMP compliant radial chromatography equipment

Micro RFC

Micro RFC columns: chromatographic performance of the radial column format in analytical scale volumes. Since the Micro RFC columns are an accurate scale-down...

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Lab HP-RFC

Lab HP-RFC columns offer “Radical” Chromatographic performance on bench scale. Ultra fast processing in robust processes with any resin and easy handling. Lab columns' suitability...
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SUPR columns

Extended range Micro to Process RFC columns in a prepacked-prevalidated format "plug-and-produce". Prepacked columns can be used for extended periods, dedicated projects...
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Pilot Process Industrial RFC

Proxcys High-Performance Radial Flow Chromatography (HP-RFC) columns are applicable from Pilot to Industrial scale operations. The "work-horses"...

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Accessories & Systems

Proxcys offers a full range of cGMP compliant accessories including sanitary Bubble traps, Intelligent packing systems and miscellaneous...
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Custom Engineering

Proxcys will develop and supply ancillary equipment that support your Downstream Processing tasks and handling. Sophisticated 3D modelling will allow fast turnaround...
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Radial Compressed© packing results in homogenous packing

Annular Port packing demonstrated in the animation below clearly demonstrates the controlled (vertical) buildup of the resin layering it gently but firmly as a perfect cylinder against the outer frit.

 

 

Annular port Radial Compressed© packing packs the radial columns with unique precision and reproducibility. This packing technique allows accurate and reproducible packing of extremely short bed heights (< 3cm !!) and tall columns (130 cm !!). Flow rate during packing is optimized for each resin.

 

Recycling packing is the most flexible packing mode, suitable for medium- to rigid resins (sepharose, polymeric). Recycling packing procedure allows a starting slurry concentrations between 50% and 80% therewith reducing the packing vessel volume to a suitable size.

 

During the recycling packing procedure the packing liquid is fed back into the packing vessel (see scheme below), causing the slurry to be diluted during packing. The compression of the resin during Radial Compressed packing is a function of Packing velocity (energy) and packing duration (compacting). Proper balance of the hydrodynamics cause the packing to be firm and homogenous. The main recycling packing control parameters are:

  • Slurry concentration
  • Packing velocity
  • Amount of settleled resin: suplus or insufficient
  • Viscosity (density) of the packing buffer

 

Effect of resin quantity and packing velocity

Graphs below show the mechanism behind recycling packing. Diluted starting slurry will increase the durstion of packing, hence allowing the resin to compact stronger. A number of protocol variants are available to make a robust reproducible S.O.P. for the packing of any column.  

 

Fig. 5: Packing process modulation

 

The Picture shows a column being packed. ~85% of the resin is packed. The arrows point to the direction the packed bed is growing (outside-in). The clear cylinder in the middle clearly shows the accuracy of the packed bed cylinder.

 

Radial Compressed© Packing Summary

  • Easy handling and set-up
  • Fast packing
  • Reproducible
  • Compatible with all resins
  • Optimized compression
  • No loss of valuable resin
  • Packing with zero excess possible

Proxcys B.V. is the world's primary producer of high performance low pressure cGMP compliant radial chromatography equipment.

Contact

Proxcys BV

Bedrijvenweg 4

NL-7833 JH Nieuw-Amsterdam

The Netherlands

Phone: +31 591 677 447

Fax: +31 591 677 448

Email: info@proxcys.com